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Registros recuperados: 62 | |
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Devin, S.; Burgeot, Thierry; Giamberini, L.; Minguez, L.; Pain-devin, S.. |
The growing need to evaluate the quality of aquatic ecosystems led to the development of numerous monitoring tools. Among them, the development of biomarker-based procedures, that combine precocity and relevance, is recommended. However, multi-biomarker approaches are often hard to interpret, and produce results that are not easy to integrate in the environmental policies framework. Integrative index have been developed, and one of the most used is the integrated biomarker response (IBR). However, an analysis of available literature demonstrated that the IBR suffers from a frequent misuse and a bias in its calculation. Then, we propose here a new calculation method based on both a more simple formula and a permutation procedure. Together, these... |
Tipo: Text |
Palavras-chave: Biomarkers; Integrated Index; Environmental risk assessment; Pollution; Water Framework Directive. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00176/28712/27276.pdf |
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Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois. |
A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during... |
Tipo: Text |
Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf |
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Mauffret, Aourell; Wessel, Nathalie; Roubeix, Vincent; Akcha, Farida; Chouvelon, Tiphaine; Aminot, Yann; Munschy, Catherine; Couteau, Jérôme; Briaudeau, Tifanie; Izagirre, Urtzi; Mahe, Kelig; Godfrin, Yoann; Pollono, Charles; Moisan, Karine; Olivier, Nathalie; Bely, Nadege; Bruzac, Sandrine; Crochet, Sylvette; Thomas, Bastien; Sireau, Teddy; Burgeot, Thierry. |
Le dispositif « SELI » est mis en place depuis 2016 pour acquérir des données sur les effets biologiques intégrateurs de la contamination chimique chez des organismes marins (poissons et bivalves) et ainsi renseigner le critère 2 du descripteur 8 de la DCSMM (Le niveau de concentration des contaminants ne provoque pas d’effets dus à la pollution). Le présent rapport concerne le volet SELIseine. Des poissons plats (194 soles, flets et limandes) ont été prélevés en septembre 2018 sur le N/O Antéa, et des moules ont été prélevées en février 2019 sur 6 stations dans le cadre du suivi ROCCH MV suivant le protocole de la convention OSPAR (2012). Les biomarqueurs suivis chez ces organismes couvrent plusieurs types d’effets toxiques (reprotoxicité, génotoxicité,... |
Tipo: Text |
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Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00688/80024/83012.pdf |
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Wessel, Nathalie; Santos, Raphael; Menard, Dominique; Le Menach, K.; Buchet, Vincent; Le Bayon, Nicolas; Loizeau, Veronique; Burgeot, Thierry; Budzinski, H.; Akcha, Farida. |
Polycylic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environment Their toxicity is mainly linked to the ability of marine species to biotransform them into reactive metabolites PAHs are thus often detected at trace levels in animal tissues For biomonitoring purposes this findings have two main consequences (i) the determination of the PAH tissue concentration is not suitable for the evaluation of individual exposure to PAHs (ii) It can explain sometimes the lack of correlations obtained with relevant markers of toxicity such as genotoxicity biomarkers The aim of the present study was to better investigate the link between PAH exposure and genotoxicity in marine flatfish During a laboratory experiment juvenile soles were exposed... |
Tipo: Text |
Palavras-chave: Ecotoxicology; Sole; PAH; Metabolites; EROD; Comet assay. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12970/10834.pdf |
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Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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Beliaeff, Benoit; Burgeot, Thierry. |
Activity of 7-ethoxyresorufin-O-deethylase (EROD) in fish is certainly the best-studied biomarker of exposure applied in the field to evaluate biological effects of contamination in the marine environment. Since 1991, a feasibility study for a monitoring network using this biomarker of exposure has been conducted along French coasts. Using data obtained during several cruises, this study aims to determine the number of fish required to detect a given difference between 2 mean EROD activities, i.e. to achieve an a priori fixed statistical power (l-beta) given significance level (alpha), variance estimations and projected ratio of unequal sample sizes (k). Mean EROD activity and standard error were estimated at each of 82 sampling stations. The... |
Tipo: Text |
Palavras-chave: Biomonitoring; Estimation; EROD activity; Statistical power; Sample size; Sampling design. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00337/44787/44483.pdf |
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Hylland, Ketil; Robinson, Craig D.; Burgeot, Thierry; Martinez-gomez, Concepcion; Lang, Thomas; Svavarsson, Jorundur; Thain, John E.; Vethaak, A. Dick; Gubbins, Mattew J.. |
This paper reports a full assessment of results from ICON, an international workshop on marine integrated contaminant monitoring, encompassing different matrices (sediment, fish, mussels, gastropods), areas (Iceland, North Sea, Baltic, Wadden Sea, Seine estuary and the western Mediterranean) and endpoints (chemical analyses, biological effects). ICON has demonstrated the use of a framework for integrated contaminant assessment on European coastal and offshore areas. The assessment showed that chemical contamination did not always correspond with biological effects, indicating that both are required. The framework can be used to develop assessments for EU directives. If a 95% target were to be used as a regional indicator of MSFD GES, Iceland and offshore... |
Tipo: Text |
Palavras-chave: ICON; Contaminants; European seas; Biological effects; Assessment. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00335/44583/44301.pdf |
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Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida. |
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore... |
Tipo: Text |
Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf |
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Caquet, Th.; Roucaute, M.; Mazzella, N.; Delmas, F.; Madigou, C.; Farcy, E.; Burgeot, Thierry; Allenou, Jean-pierre; Gabellec, Raoul. |
A 2-year study was implemented to characterize the contamination of estuarine continuums in the Bay of Vilaine area (NW Atlantic Coast, Southern Brittany, France) by 30 pesticide and biocide active substances and metabolites. Among these, 11 triazines (ametryn, atrazine, desethylatrazine, desethylterbuthylazine, desisopropyl atrazine, Irgarol 1051, prometryn, propazine, simazine, terbuthylazine, and terbutryn), 10 phenylureas (chlortoluron, diuron, 1-(3,4-dichlorophenyl)-3-methylurea, fenuron, isoproturon, 1-(4-isopropylphenyl)-3-methylurea, 1-(4-isopropylphenyl)-urea, linuron, metoxuron, and monuron), and 4 chloroacetanilides (acetochlor, alachlor, metolachlor, and metazachlor) were detected at least once. The objectives were to assess the corresponding... |
Tipo: Text |
Palavras-chave: Estuary; Herbicides; Booster biocides; Species sensitivity distribution; Risk quotient; AtlanticCoast. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23571/22554.pdf |
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Burgeot, Thierry; Bocquene, Gilles; Truquet, Philippe; Le Dean, Loic; Poulard, Jean-charles; Dorel, Didier; Souplet, Arnauld; Galgani, Francois. |
An oceanographic cruise in 1991 along the English Channel and Atlantic coasts of France indicated the value of dragonet Callionymus lyra Linnaeus, 1758 as a target species for monitoring biological effects. Ethoxyresorufin-O-deethylase measurements in this species provided a first assessment of the effects of hydrocarbons (PCB, PAH, dioxins) on the marine environment. The specific behavior of dragonet was defined by biochemical characterization. Biological data were obtained during 3 additional oceanographic cruises to study changes in the abundance and distribution of the species in the English Channel and the Bay of Biscay. This first assessment, carried out in the context of the National Observation Network, indicated that pollutants do not have... |
Tipo: Text |
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Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00091/20243/17895.pdf |
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Moreau, Pierrick; Faury, Nicole; Burgeot, Thierry; Renault, Tristan. |
Since 2008, mass mortality outbreaks have been reported in all French regions producing Pacific oysters, and in several Member States of the European Union. These mass mortality events of Pacific oysters are related to OsHV-1 infection. They occur during spring and summer periods leaving suspect the quality of the marine environment and the role of seasonal use of pesticides associated with the arrival of freshwater in oyster rearing areas. Pesticides have been also detected in French coastal waters, especially in areas of oyster production. Using PMA real-time PCR we showed that a mixture of 14 pesticides has no effect on the integrity of virus capsids from viral suspension in the conditions tested. A contact of oysters with this pesticide mixture was... |
Tipo: Text |
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Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00273/38405/36730.pdf |
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Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry. |
Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation... |
Tipo: Text |
Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf |
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Masson, Daniel; Ratiskol, Gilles; Burgeot, Thierry; Margat, Sylvie. |
Les secteurs conchylicoles atteints par les efflorescences phytoplanctoniques toxiques sont fermés par l'Administration dès que le taux de contamination devient dangereux pour la consommation humaine. Cette fermeture peut durer plusieurs mois empêchant les conchyliculteurs de commercialiser leurs produits, ce qui a des conséquences économiques parfois graves. Il n'existe jusqu'à présent aucune possibilité technique de décontaminer ces coquillages en grande quantité et dans des délais économiquement viables. L'étude entreprise visait à mettre au point une technique permettant aux professionnels de décontaminer des coquillages rapidement (quinze jours au moins) en milieu contrôlé (bassins). L'hypothèse testée était que des coquillages placés dans un milieu... |
Tipo: Text |
Palavras-chave: Coquillages; Décontamination; Toxicité; Phytoplancton. |
Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00134/24499/22527.pdf |
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Amiard-triquet, Claude; Burgeot, Thierry; Claisse, Didier. |
Monitoring of marine environment quality can be based on chemical analysis of contaminants in the major components of the environment: seawater, sediment, biota. The respective interest of these different matrices is discussed and the fundamentals for the use of bivalves in the international Mussel Watch are presented. The objectives of the French RNO are 1) to assess the levels and trends of contaminants; 2) to assess the biological effects of contaminants. Analysis of the chemicals in biota is an indispensable complement to the biological approach, allowing their bioavailable fraction to be quantified. However, only a fraction of xenobiotics incorporated in living beings is biologically active and can exert a noxious effect on the biota. In addition,... |
Tipo: Text |
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Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00017/12848/9793.pdf |
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Galgani, Francois; Bocquene, Gilles; Truquet, Philippe; Burgeot, Thierry; Chiffoleau, Jean-francois; Claisse, Didier. |
The establishment of a network to assess pollutant effects is a current concern of international bodies such as the North Sea Task Force, the International Council for the Exploration of the Sea and the Intergovernmental Oceanographic Commission. With respect to the French coasts, three major considerations have been defined : the choice of monitoring areas ; the determination of a target species according to different criteria such as wide distribution, limited migration and contact with pollutant-rich sediments; and the selection of suitable parameters. This paper concerns the strategy adopted for the French coast. Ethoxyresorufin-O-deethylase, a specific cytochrome P450-dependent monooxygenase, measured by a simple microplate assay, was selected as an... |
Tipo: Text |
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Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00100/21169/18787.pdf |
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Quiniou, Francoise; Blanchard, Michel; Bocquene, Gilles; Burgeot, Thierry; Giboire, Louis; His, Edouard; Le Du, Anne. |
La présente étude a pour objectif de sélectionner des méthodes de laboratoire et de terrain les mieux appropriées pour l'évaluation de l'écotoxicité de sédiments dragués : indicateurs biochimiques d'exposition (EROD et AChE), tests de toxicité sublétale (micro-organismes, algues, embryons de bivalve et extrait enzymatiques de poisson), indices physiologiques de bivalve (respiration, excrétion, mouvements valvaires). Une synthèse des résultats est présentée accompagnée, en annexes, des rapports des différents participants à l'étude. |
Tipo: Text |
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Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00104/21573/19153.pdf |
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Mauffret, Aourell; Wessel, Nathalie; Akcha, Farida; Munschy, Catherine; Chouvelon, Tiphaine; Couteau, Jérôme; Briaudeau, Tifanie; Izagirre, Urtzi; Aminot, Yann; Pollono, Charles; Moisan, Karine; Olivier, Nathalie; Bely, Nadege; Crochet, Sylvette; Thomas, Bastien; Sireau, Teddy; Menard, Dominique; Burgeot, Thierry. |
Le dispositif « SELI » est mis en place depuis 2016 pour acquérir des données sur les effets biologiques intégrateurs de la contamination chimique chez des organismes marins (poissons et bivalves) et ainsi renseigner le critère 2 du descripteur 8 de la DCSMM (Le niveau de concentration des contaminants ne provoque pas d’effets dus à la pollution). Des soles ont été prélevées en août et septembre 2017 et des moules en février 2018, dans le cadre du suivi ROCCH MV. Les biomarqueurs suivis chez ces organismes couvrent plusieurs mécanismes d’actions toxiques (reprotoxicité, génotoxicité, neurotoxicité) et incluent des mesures de la santé générale de l'organisme. Les contaminants suivis sont des éléments traces métalliques et des contaminants organiques... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00620/73215/72410.pdf |
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Registros recuperados: 62 | |
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